美國紫外交聯儀Stratalinker UV 2400 庫號:M216912
美國紫外交聯儀Stratalinker UV 2400 庫號:M216912
美國紫外交聯儀Stratalinker UV 2400 庫號:M216912
特點:
為了使核苷酸固定在膜上,傳統的方法是將膜置于真空烘箱中80度烘2小時,在紫外交聯儀上僅需30-60秒
與真空烘焙相比,大大增加雜交信號強度
內部配備光度檢測儀,可以補償由于紫外燈泡老化造成的輸出偏移
具自動交聯、時間和能量三種模式供選擇
帶有顯示時間、能量的顯示器及設置面板
應用:
將核酸共價結合到固相支持物(如硝酸纖維素膜)上
Northem,Southern,dot及slot印跡分析
雜交芯片的制備
菌落、噬菌斑篩選,瓊脂糖凝膠中DNA的切割
產生胸腺二聚體,用于部分限制性內切酶消化作圖
紫外敏感性測試,用于鑒定recA宿主菌
樣品紫外照射處理
誘導培養細胞的凋亡
照射波長:254nm(配8瓦254nm燈泡5個)
內部尺寸:47.7W/34.3D/16.8H
Stratagene UV STRATAl
inkER 2400 UV Crossl
inker
Agilent Genomics / Stratagene Stratal
inker 2400 UV Crossl
inker
Features
For Crossl
inking DNA or RNA to Coated Glass Slides, Nylon, Nitrocellulose or Nylon-Reinforced Nitrocellulose Membranes
Greatly Reduces Valuable Time From Several Oven Baking Hours to 30-60 Seconds
Increases Hybridization Signal Compared to Oven Baking
Internal Photodetector Compensates for Shifting Output of Aging UV Bulbs
Can be Operated in Autocrossl
ink, Time or Energy Modes
Des
cription
The Agilent Genomics / Stratagene UV crossl
inker is designed to crossl
ink DNA or RNA to nylon, nitrocellulose, or nylon-reinforced nitrocellulose membranes. The crossl
inking process takes o
nly 25-50 seconds, in co
ntrast to the traditio
nal method of baking filters at 80for 2 hours. Additionally, crossl
inking has been shown to significantly increase hybridization signals when compared to oven-baking. For optimal crossl
inking performance, each Stratal
inker UV crossl
inker is equipped with an internal photodetector designed to compensate for the natural shift in power output of aging ultraviolet bulbs.
The Stratal
inker UV crossl
inker may be used for Northern, Southern, dot or slot blot analysis,1-3 co
lony or plaque screening, nicking of DNA in agarose gels prior to blotting,4 dimer formation to perform partial digests for rapid restriction mapping,5 UV sensitivity testing for host strain verification,6 and UV irradiation of PCR samples.7 The Stratal
inker UV crossl
inker may be used in the Autocrossl
ink, Time, or Energy modes, and the apparatus is available in two co
nvenient sizes-the Stratal
inker 2400 UV crossl
inker is for larger membranes.

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