中文名稱    酪氨酸蛋白激酶受體B6抗體    

英文名稱    EphB6    

別    名    EPH receptor B6; EphB6; EPHB6_HUMAN; Ephrin type B receptor 6 precursor; Ephrin type-B receptor 6; HEP; MGC129910; MGC129911; Tyrosine protein kinase defective receptor EPH 6; Tyrosine-protein kinase-defective receptor EPH-6.    

供 應 商    遠慕生物

研究領域    免疫學  發育生物學  神經生物學  信號轉導  生長因子和激素  激酶和磷酸酶  細胞類型標志物      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Dog, Pig, Horse,     

產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 IF=1:100-500 酪氨酸蛋白激酶受體B6抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    111kDa    

細胞定位    細胞膜 分泌型蛋白     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human EphB6    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

酪氨酸蛋白激酶受體B6抗體產品介紹    background:

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ephrin receptor encoded by this gene lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands. [provided by RefSeq, Jul 2008].

Function:
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T-cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2.

Subunit:
Interacts with CBL and EPHB1. Interacts with FYN; this interaction takes place in a ligand-independent manner.

Subcellular Location:
Membrane; Single-pass type I membrane protein. Isoform 3: Secreted.

Tissue Specificity:
Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues.

Post-translational modifications:
Ligand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion.

Similarity:
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.

Gene ID:
2051

Database links:

Entrez Gene: 2051 Human

Entrez Gene: 13848 Mouse

Entrez Gene: 312275 Rat

Omim: 602757 Human

SwissProt: O15197 Human

SwissProt: O08644 Mouse

SwissProt: P0C0K7 Rat

Unigene: 380089 Human

Unigene: 271976 Mouse

Unigene: 125930 Rat


important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.