使用目的：本試劑盒用于測(cè)定進(jìn)口/國(guó)產(chǎn)大鼠血清、血漿及相關(guān)液體樣本補(bǔ)體片斷3a（C3a）含量。 

本試劑僅供研究使用      標(biāo)本：血清或血漿

注：本產(chǎn)品僅供科研使用！

The performance of kit:

1 sensitivity: minimum detection concentration is less than 1 standard. LiC3aarity of dilution. Sample liC3aar regression and the expected concentration correlation coefficient R value is 0.990.

2: no specific reaction with other cytokiC3as.

3 repeaC3aility: plate, plate between the coefficients of variation were less than 10%.

 試驗(yàn)原理：

C3a試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知C3a濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測(cè)。先將C3a和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后，加入親和素標(biāo)記過(guò)的HRP。再經(jīng)過(guò)溫育和洗滌，去除未結(jié)合的酶結(jié)合物，然后加入底物A、B，和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中C3a的濃度呈比例關(guān)系。

補(bǔ)體片斷3a試劑盒,elisa試劑盒說(shuō)明書,ELISA試劑盒,ELISA試劑盒,進(jìn)口/國(guó)產(chǎn)大鼠elisa試劑盒說(shuō)明書