使用目的：本試劑盒用于測定進(jìn)口/國產(chǎn)小鼠血清、血漿及相關(guān)液體樣本白介素10（NGAL）含量。 

本試劑僅供研究使用      標(biāo)本：血清或血漿

注：本產(chǎn)品僅供科研使用！

The performance of kit:

1 sensitivity: minimum detection concentration is less than 1 standard. LiNGALarity of dilution. Sample liNGALar regression and the expected concentration correlation coefficient R value is 0.990.

2: no specific reaction with other cytokiNGALs.

3 repeaNGALility: plate, plate between the coefficients of variation were less than 10%.

 試驗(yàn)原理：

NGAL試劑盒是固相夾心法酶聯(lián)免疫吸附實(shí)驗(yàn)（ELISA）.已知NGAL濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將NGAL和生物素標(biāo)記的抗體同時(shí)溫育。洗滌后，加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌，去除未結(jié)合的酶結(jié)合物，然后加入底物A、B，和酶結(jié)合物同時(shí)作用。產(chǎn)生顏色。顏色的深淺和樣品中NGAL的濃度呈比例關(guān)系。

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