ATCC® Number: CCL-10?
Designations: BHK-21 [C-13]
Depositors: I Macpherson
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mesocricetus auratus (hamster, Syrian golden)
Morphology: fibroblast
Source: Organ: kidney
Disease: normal
Cell Type: fibroblast
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Isolation: Isolation date: March, 1961
Applications: testing [92346] [92389]
transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Virus Susceptibility: Human adenovirus 25
Reovirus 3
Vesicular stomatitis virus
Human poliovirus 2
Reverse Transcript: negative
Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 44. This is a pseudodiploid line with the tetraploidy occurring at 4%. The karyotype is 44,XY,-6,-15,6q+,15q+ in a majority of cells analyzed. The markers 6q+ and 15q+ occurred in most cells. An occasional monosomic or trisomic condition for a normal chromosome was also detected.
Age: 1 day old newborn
HeLa Markers: N
Comments: The parent line of BHK-21(C-13) was derived from the kidneys of five unsexed, 1-day-old hamsters in March, 1961, by I.A. Macpherson and M.G.P. Stoker. [21411]
Following 84 days of continuous cultivation, interrupted only by an 8-day preservation by freezing, clone 13 was initiated by single-cell isolation. [25963]
This line has been used as a host for transformation withexpressionvectors containing selectable and amplifiable marker DNAs (e.g., Factor VIII, see ATCC CRL-8544).
The World Organization for Animal Health (OIE) uses the cells for routine diagnosis of rabies. (see: http://www.oie.int/Eng/Normes/Mmanual/A_00044.htm).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
Bioreactive Factors: Growth Factors: T cell growth factor (TCGF)
References: 2: Drayna D, et al. Genetic mapping and diagnosis of haemophilia A achieved through a BclI polymorphism in the factor VIII gene. Nature 314: 738-740, 1985. PubMed:
3: Kazazian HH Jr., et al. Restriction site polymorphism in the phosphoglycerate kinase gene on the X chromosome. Hum. Genet. 66: 217-219, 1984. PubMed:
21411: Macpherson I, Stoker M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology 16:
21412: . . Virology 14: 359-370, 1961.
25963: Macpherson I. Characteristics of a hamster cell clone transformed by polyoma virus. J. Natl. Cancer Inst. 30: 795-815, 1963.

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32473: Hussain MA, et al. POU domain transcription factor brain 4 confers pancreatic alpha-cell-specificexpressionof the proglucagon gene through interaction with a novel proximal promoter G1 element. Mol. Cell. Biol. 17: 7
32475: You M, et al. ch-IAp1, a member of the inhibitor-of-apoptosis protein family, is a mediator of the antiapoptotic activity of the v-Rel oncoprotein. Mol. Cell. Biol. 17: 7328-7341, 1997. PubMed:
32501: Jelachich ML, Lipton HL. Theiler's murine encephalomyelitis virus kills restrictive but not permissive cells by apoptosis. J. Virol. 70: 6856-6861, 1996. PubMed:
32512: Schnell MJ, et al. The minimal conserved transcription stop-start signal promotes stableexpressionof a foreign gene in vesicular stomatitis virus. J. Virol. 70: 2318-2323, 1996. PubMed:
32524: Chang YE, et al. Properties of the protein encoded by the UL32 open readingframeof herpes simplex virus 1. J. Virol. 70: 3938-3946, 1996. PubMed:
92346: Biological evaluation of medical devices. Part 5: Tests for in vitro cytotoxicity. Sydney, NSW, Australia:Standards Australia;Standards Australia AS ISO 10993.5-2002.
92389: Biological evaluation of medical devices--Part 5: Tests for in vitro cytotoxicity. Geneva (Switzerland):International Organization for Standardization/ANSI;ISO ISO 10993-5:1999.