Platinum Retroviral Expression SystemsRetroviral vectors are useful

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Platinum Retroviral Expression Systems

Retroviral vectors are useful for delivering genes of

interest into a host cell where integration into the ge-

nome is desired. However, traditional retroviral ex-

pression technologies usually result in low viral titers,

making gene expression studies difficult.

 

Our Platinum Retroviral Expression Systems incorpo-

rate superior packaging cell lines and vector technolo-

gies to produce high-titer virus with a single plasmid

transfection. Each Platinum Expression System in-

cludes one of our exclusive Platinum Packaging Cell

Lines which contain the gag and pol genes. In the

Ecotropic and Amphotropic systems, the packaging

cells also contain the envelope protein. Simply clone

your gene of interest into the vector provided and

transfect into the Platinum cells. If you choose a Pan-

tropic system, simply co-transfect with the VSV-G

plasmid provided. 

 

Not sure which Platinum Expression System is right

for you? See the previous page for a selection guide

based on the host species of your target cell.

 

 

 

•   Higher Viral Yields: Average titer 10⁷ infec-

tious units/mL with transient transfection

•   Versatile: 3 Packaging cell lines for use with

nearly any target host species

VIRAL EXPRESSION

 

 

Retrovirus Production Using the Platinum Retroviral Expres-

sion Systems.

 

Are you working with stem cells? See page 24 for our Platinum Retroviral Expression Sys-

tems specifically designed for use with ES, EC and hematopoietic stem cells.

 

 

Catalog Number

 

VPK-300

VPK-301

 

Packaging Cell Line

 

Plat-E (Ecotropic)

Plat-A (Amphotropic)

 

Shuttle Vector

 

pMXs-Puro

pMXs-Puro

 

Envelope Vector

 

———

———

 

Control Vector

 

pMX-GFP

pMX-GFP

VPK-302

Plat-GP (Pantropic)

pMXs-Puro

pCMV-VSV-G pMX-GFP

Components of the Platinum Retroviral Expression Systems.

 

Product Name 

Platinum Retroviral Expression System, Ecotropic 

Platinum Retroviral Expression System, Amphotropic 

Platinum Retroviral Expression System, Pantropic 

 

 

Size

1 kit

1 kit

1 kit

 

 

Catalog Number

VPK-300

VPK-301

VPK-302

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